Abstract:
Preeclampsia (PE) remains the major cause for
maternal and foetal mortality and morbidity all over the
world. Preeclampsia is associated with maternal, placental
aggravated inflammatory response and generalized
endothelial damage. AnnexinA1 (AnxA1) is glucocorticoid
regulated protein regulates a wide range of cellular and
molecular steps of the inflammatory response and is
implicated in resolution of inflammation. Galectin-3 (Gal3), b-galcotoside-binding lectin participates in many
functions, both intra- and extracellularly. Recently it has
been shown that galectin-3 modulates the inflammation.
Role of AnxA1 and Galectin-3 is poorly studied in context
with human reproductive disease like Preeclampsia.
Therefore, the present study examined the expression of
AnxA1 and Gal-3 which are involved in modulation of
inflammation and their association in the placental bed of
pregnancy with and without PE. The study group consisted
of placental bed biopsy tissues obtained from pregnancies
with PE (n = 30) and without (n = 30) PE. The expression
of AnxA1 and Gal-3 in the placental bed tissues was
evaluated quantitatively using Immunohisto-chemistry
(IHC), western blot and mRNA expression analysis by
quantitative RT-PCR. Our IHC, western blot and RT PCR
analyses showed the increase in the expression of AnxA1
and Gal-3 in PE group compared with the normotensive
control group (P\ 0.001). The increased expression of
AnxA1 and Gal-3 in placental bed may be associated with
a systemic inflammatory response in PE, suggesting role of
AnxA1 and Gal-3 in PE pathogenesis.
