Genetic study of nuclear factor kappa b1 gene(nfkb1)polymorphism in acute coronary syndrome

Abstract

INTRODUCTION: Most prevalent cardiovascular condition in adults,Acute Coronary Syndrome (ACS), continue to be main source of death with morbidity worldwide. In ACS multiple environmental and genetic factors plays role.Nuclear factor kappa-B gene (NFKB), transcription factor, locates at chromosome 4q24, regulating expression of proinflammatory genes linked to atherosclerosis. This study is carried out to determine relation between NFKB1 gene polymorphism in patients of ACS. AIM: To study genetic polymorphism of Nuclear factor kappa B 1 gene in patients with acute coronary syndrome. MATERIALS AND METHODS: This was Prospective cross-sectional study done in BLDE (DU), Vijayapura, Karnataka, India, in 100 patients with ACS, 8 patients were excluded and 92 patients were included who underwent clinical examination, biochemical profiles, electrocardiography, blood samples were collected and analysed for NFKB1 gene polymorphism using PCR technique. Obtained data was entered into Microsoft excel sheet for analysis following which data was analysed statistically. Results were presented as Mean (Median) ±SD, counts and percentages, and diagrams. RESULTS: This study male patients were 55 and female patients 37, commone stage group of patients were between 61-70 years, presented with chest pain ,dyspnea, risk factors included diabetes,hypertension, smoking was least common risk factor. Commonest ECG findingwas NSTEMI. This study found no mutation in exon 10 and 2 of NFKB1 gene in patients of acute coronary syndrome. CONCLUSION: This study found no pathogenic mutation in exon 10 and 2 of NFKB1 gene in patients of acute coronary syndrome implying more research to be carried out with other pathogenic genomes and regulatory factor.